What folder? You can just move the Spine installation folder anywhere you want. If it's the Spine folder is your user folder, then you would need to change in Windows where your user folder is at. However, you can delete any temp, cache, and update folders folders found in your Spine user folder. You can also delete the backups folder, though it contains saves of older versions of your projects which can come in handy in a disaster. The rest of the files in your Spine user folder are very small.